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goat anti lrig1 af3688 antibody  (R&D Systems)


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    Structured Review

    R&D Systems goat anti lrig1 af3688 antibody
    Goat Anti Lrig1 Af3688 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+lrig1/Mouse+LRIG1+Antibody/pm41533906-54-0-7
    Average 94 stars, based on 54 article reviews
    goat anti lrig1 af3688 antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Lgr5+ Stem Cells Maintain Apex Position in Cell Hierarchy of the Intestinal Epithelium During Homeostasis and Injury
    Article Snippet: .. Sections were incubated overnight with primary antibodies targeting the following markers: mouse monoclonal anti-Ki67 (BD Biosciences, Cat# 55600), goat polyclonal anti-GFP (Abcam, Cat# ab5450), rabbit monoclonal anti-Olfm4 (Cell Signaling Technology, Cat# 39141S), goat anti-Sca1Ly6 (R&D Systems, Cat# AF1226-SP), goat anti-Lrig1 (R&D Systems, Cat# AF3688), rabbit anti-Fzd5 (Abcam, Cat# ab75234) and rabbit polyclonal anti-lysozyme (Dako, Cat# EC32117). .. After primary antibody incubation, secondary antibodies conjugated with DyLight fluorophores (Fisher Scientific) were applied, and sections were counterstained with DAPI (Sigma-Aldrich) for 30 minutes.

    Article Title: Investigation of Novel c-Kit-expressing Smooth Muscle Cells in Murine Cecum
    Article Snippet: .. After preincubation with normal donkey serum (5% in PBS) for 1 hr, sections were incubated with the following antibodies at room temperature: goat anti-c-Kit (AF1356, R&D, 1:1000), rat anti-c-Kit (ACK4, CL8936AP, Cedarlane, 1:500), mouse anti-α-smooth muscle actin-Cy3 (1A4, C6198, Sigma, 1:3000), rat anti-smooth muscle myosin heavy chain (KM3669, 80058, Yamasa, 1:1000), rabbit anti-PGP9.5 (RA95101, UltraClone, 1:5000), rabbit anti-TMEM16A (ab53212, Abcam, 1:500), and goat anti-LRIG1 (AF3688, R&D, 1:1000) [ , ]. .. After sections were incubated overnight with primary antibodies, they were washed with PBS before incubation in the secondary antibody (Alexa Fluor-coupled donkey anti-IgG, Molecular Probes, 1:500) for 1 hr at room temperature.

    Article Title: Urban Particulate Matter Triggers Meibomian Gland Dysfunction
    Article Snippet: Sagittal and coronal sections of MG tissues were permeabilized in 0.2% Triton X-100 and then blocked with PBS containing 3% BSA (Sigma-Aldrich, St Louis, MO, USA). .. The primary antibodies used in this step of overnight incubation included rabbit anti-Ki67 (1:150, MA5-14520; Invitrogen), rabbit anti-P63 (1:100, ab124762; Abcam), goat anti-Lrig1 (1:100, AF3688; R&D Systems, Minneapolis, MN, USA), rabbit anti-NLRP3 (1:100, NBP2-12446; R&D Systems), rabbit anti-cleaved caspase-1 (1:100, A0964; Abclonal), rabbit anti-gasdermin D (GSDMD) (1:100, A18281; Abclonal), rabbit anti-NE (1:100, A13015; Abclonal), and mouse anti-cytokeratin 6 (K6) (1:100, ab18586; Abcam) for sagittal sections, whereas rabbit anti-cytokeratin 1 (K1) (1:50, ab185628; Abcam), rabbit anti-cytokeratin 10 (K10) (1:50, ab76318; Abcam) for coronal sections. .. The following fluorophore-conjugated secondary antibodies were used for the subsequent incubation of the slides in the dark at room temperature for 1 hour: Alexa Fluor 488– or Alexa Fluor 555–labeled donkey anti-rabbit IgG antibody (1:500, 488-ab150073, 555-ab155062; Abcam), Alexa Fluor 647–labeled donkey anti-goat IgG antibody (1:500, ab150135; Abcam), and Alexa Fluor 488–labeled donkey anti-mouse IgG antibody (1:500, ab150105; Abcam).

    Article Title: Suppression of intestinal tumors by targeting the mitotic spindle of intestinal stem cells.
    Article Snippet: Immunofluorescence analysis of organoids Organoids cultured in 48-well plates were washed once with PBS, fixed in 4% paraformaldehyde for 30 min and transferred into 1.5 ml Eppendorf tubes. .. After permeabilization with PBS containing 0.1% Triton X-100 (PBST) followed by blocking with 10% goat or donkey serum for 1 h at room temperature, the organoids were incubated overnight at 4 °C with the following antibodies: goat anti-EphB2 (1:200, R&D Technologies), mouse anti-β-catenin (1:200, BD Transduction), mouse anti-α-tubulin (1:200, Sigma), rabbit anti-phospho-histone H3 (ser10) (1:500, Millipore, Darmstadt, Germany), rabbit anti-lysozyme (1:600, DAKO), goat anti-Lrig1 (1:100, R&D), and mouse anti-ALDH1 (1:100, Abcam). .. The rabbit anti-TACC3 and rabbit anti-TOGp antibodies were previously described.21 After extensive washes with PBST, secondary antibodies (1:200, Jackson ImmunoResearch) were applied with 4’,6-diamidino-2-phenylindole for 2 h at room temperature.

    Blocking Assay:

    Article Title: Suppression of intestinal tumors by targeting the mitotic spindle of intestinal stem cells.
    Article Snippet: Immunofluorescence analysis of organoids Organoids cultured in 48-well plates were washed once with PBS, fixed in 4% paraformaldehyde for 30 min and transferred into 1.5 ml Eppendorf tubes. .. After permeabilization with PBS containing 0.1% Triton X-100 (PBST) followed by blocking with 10% goat or donkey serum for 1 h at room temperature, the organoids were incubated overnight at 4 °C with the following antibodies: goat anti-EphB2 (1:200, R&D Technologies), mouse anti-β-catenin (1:200, BD Transduction), mouse anti-α-tubulin (1:200, Sigma), rabbit anti-phospho-histone H3 (ser10) (1:500, Millipore, Darmstadt, Germany), rabbit anti-lysozyme (1:600, DAKO), goat anti-Lrig1 (1:100, R&D), and mouse anti-ALDH1 (1:100, Abcam). .. The rabbit anti-TACC3 and rabbit anti-TOGp antibodies were previously described.21 After extensive washes with PBST, secondary antibodies (1:200, Jackson ImmunoResearch) were applied with 4’,6-diamidino-2-phenylindole for 2 h at room temperature.

    other:

    Article Title: Control of Murine Brown Adipocyte Development by GATA6
    Article Snippet: Goat anti-LRIG1 , R and D Systems, AF3688 , RRID:AB_2138836.

    Article Title: An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis.
    Article Snippet: In-house and commercially available antibodies were used (below). chicken anti-Krt14 (Covance, SIG2376, 1:500), mouse anti-Krt14 (LL002, in house, 1:200); directly conjugated (AlexaFluor 488) Krt15 (LHK-15, in-house, 1:50); human anti-p63 (SCBT, sc367333, 1:100); rabbit anti-filaggrin (Covance, PRB-417P, 1:100); mouse anti-FASN (SCBT, sc48357, 1:100); rabbit anti-Ki67 (Novocastra, NCL-Ki67p, 1:500); rabbit anti-Ki67 (abcam, ab16667, 1:500); rabbit anti- Phospho-S6 Ribosomal Protein (Ser235/236) (pS6K, Cell signaling, 2211, 1:200); rabbit anti-PCadherin (Cell signaling, 2130, 1:200); rabbit anti-Vimentin (Cell signaling, 5741s, 1:500); rabbit anti-K10 (Covance, PRB-159P, 1:500); FITC conjugated rat anti-CD49f (Integrin α6, Biolegend, 313606, 1:100); goat anti-Lrig1 (R&D Systems, FAB3688G, 1:200); rabbit anti-Scd1 (Cell signaling, 2794s, 1:500); mouse anti-involucrin (SY5, in-house, 1:500) mouse anti-Pankeratin (abcam, ab8068, 1:200);rat anti-CD34 (RAM34, Thermo Fisher, 14-0341-82, 1:200); Rabbit anti-Phospho-4EBP1 (Thr37/46) (Cell Signalling, 236B4, 1:500).



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